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mini trans blot electrophoretic transfer cell apparatus  (Bio-Rad)


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    Bio-Rad mini trans blot electrophoretic transfer cell apparatus
    Mini Trans Blot Electrophoretic Transfer Cell Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 13033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+trans+blot+electrophoretic+transfer+cell+system/Trans-Blot+Turbo+Transfer+System/pm42112905-325-10-16
    Average 99 stars, based on 13033 article reviews
    mini trans blot electrophoretic transfer cell apparatus - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    SDS Page:

    Article Title: A protective cGAMP-mediated anti-tumor immune response can proceed without LRRC8/VRAC channels
    Article Snippet: .. Equal amounts of protein were separated by SDS-PAGE on a 7.5% polyacrylamide gel and transferred to a 0.45 μm nitrocellulose membrane (Cytiva, 10600002) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad, 1703930). .. Protein concentrations were determined by BCA assay (Thermo Fisher, A55860).

    Article Title: A protective cGAMP-mediated anti-tumor immune response can proceed without LRRC8/VRAC channels.
    Article Snippet: Culture medium was removed and cells were incubated for 3 h in isotonic (302 mOsm/L; 85 mM mannitol, 90 mM NaCl, 1 mM MgCl 2 , 2 mM CaCl 2, 4 mM KCl, 10 mM glucose, 10 mM HEPES pH 7.4) or hypotonic (217 mOsm/L; 90 mM NaCl, 1 mM MgCl 2 , 2 mM CaCl 2, 4 mM KCl, 10 mM glucose, 10 mM HEPES pH 7.4) solution containing 10 μg/ml 2 ′ 3 ′ -cGAMP (InvivoGen, tlrl-nacga23– 02). .. Equal amounts of protein were separated by SDS-PAGE on a 7.5% polyacrylamide gel and transferred to a 0.45 μm nitrocellulose membrane (Cytiva, 10600002) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad, 1703930). .. Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% Triton-X100 and 0.5% deoxycholate) supplemented with protease inhibitors cOmplete (Merck, 11836145001) and Pefabloc (Roth, A154.3).

    Membrane:

    Article Title: A protective cGAMP-mediated anti-tumor immune response can proceed without LRRC8/VRAC channels
    Article Snippet: .. Equal amounts of protein were separated by SDS-PAGE on a 7.5% polyacrylamide gel and transferred to a 0.45 μm nitrocellulose membrane (Cytiva, 10600002) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad, 1703930). .. Protein concentrations were determined by BCA assay (Thermo Fisher, A55860).

    Article Title: A protective cGAMP-mediated anti-tumor immune response can proceed without LRRC8/VRAC channels.
    Article Snippet: Culture medium was removed and cells were incubated for 3 h in isotonic (302 mOsm/L; 85 mM mannitol, 90 mM NaCl, 1 mM MgCl 2 , 2 mM CaCl 2, 4 mM KCl, 10 mM glucose, 10 mM HEPES pH 7.4) or hypotonic (217 mOsm/L; 90 mM NaCl, 1 mM MgCl 2 , 2 mM CaCl 2, 4 mM KCl, 10 mM glucose, 10 mM HEPES pH 7.4) solution containing 10 μg/ml 2 ′ 3 ′ -cGAMP (InvivoGen, tlrl-nacga23– 02). .. Equal amounts of protein were separated by SDS-PAGE on a 7.5% polyacrylamide gel and transferred to a 0.45 μm nitrocellulose membrane (Cytiva, 10600002) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad, 1703930). .. Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% Triton-X100 and 0.5% deoxycholate) supplemented with protease inhibitors cOmplete (Merck, 11836145001) and Pefabloc (Roth, A154.3).

    Article Title: Protocol for large-scale, high-yield, high-purity extracellular vesicle purification from human plasma.
    Article Snippet: .. Run the gel electrophoresis by following the same protocol as reported previously (in step 32b). c. Using Biorad Mini Trans-Blot Electrophoretic Transfer Cell system (includes two gel holder cassettes, foam pads, electrodes, tank, blue cooling unit, lid with cables) and a Biorad PowerPacTM Basic Power Supply, perform wet transfer of the gel onto a Nitrocellulose membrane at a constant voltage of 30V at 4 ◦ C for 24 h (or 120V for 2 h) in a tank filled with the 1X Transfer Buffer working solution, which is freshly made by mixing 700 mL DI water and 200 mL methanol with 100 mL of 10X Transfer Buffer stock solution (refer to “materials and equipment’’ for composition). d. Block the transferred membrane with 5% non-fat milk or 5% BSA in 1X TBST for 1 h on rotation. e. Discard the blocking solution and incubate the membranes with primary antibody for 24 h at 4 ◦ C. f. The following day, wash the membranes with 1X TBST 3 times for 5 min each. ..

    Article Title: Protocol for large-scale, high-yield, high-purity extracellular vesicle purification from human plasma
    Article Snippet: .. Run the gel electrophoresis by following the same protocol as reported previously (in step 32b). c. Using Biorad Mini Trans-Blot Electrophoretic Transfer Cell system (includes two gel holder cassettes, foam pads, electrodes, tank, blue cooling unit, lid with cables) and a Biorad PowerPacTM Basic Power Supply, perform wet transfer of the gel onto a Nitrocellulose membrane at a constant voltage of 30V at 4°C for 24 h (or 120V for 2 h) in a tank filled with the 1X Transfer Buffer working solution, which is freshly made by mixing 700 mL DI water and 200 mL methanol with 100 mL of 10X Transfer Buffer stock solution (refer to “ ” for composition). d. Block the transferred membrane with 5% non-fat milk or 5% BSA in 1X TBST for 1 h on rotation. e. Discard the blocking solution and incubate the membranes with primary antibody for 24 h at 4°C. f. The following day, wash the membranes with 1X TBST 3 times for 5 min each. ..

    other:

    Article Title: An orally active, small-molecule TNF inhibitor that disrupts the homotrimerization interface improves inflammatory arthritis in mice.
    Article Snippet: Excessive signaling by the proinflammatory cytokine TNF is involved in several autoimmune diseases, including rheumatoid arthritis (RA).. However, unlike the approved biologics currently used to treat this and other conditions, commercially available small-molecule inhibitors of TNF trimerization are cytotoxic or exhibit low potency.. Here, we report a TNF-inhibitory molecule (TIM) that reduced TNF signaling in vitro and was an effective treatment in a mouse model of RA.

    Nucleic Acid Electrophoresis:

    Article Title: Protocol for large-scale, high-yield, high-purity extracellular vesicle purification from human plasma.
    Article Snippet: .. Run the gel electrophoresis by following the same protocol as reported previously (in step 32b). c. Using Biorad Mini Trans-Blot Electrophoretic Transfer Cell system (includes two gel holder cassettes, foam pads, electrodes, tank, blue cooling unit, lid with cables) and a Biorad PowerPacTM Basic Power Supply, perform wet transfer of the gel onto a Nitrocellulose membrane at a constant voltage of 30V at 4 ◦ C for 24 h (or 120V for 2 h) in a tank filled with the 1X Transfer Buffer working solution, which is freshly made by mixing 700 mL DI water and 200 mL methanol with 100 mL of 10X Transfer Buffer stock solution (refer to “materials and equipment’’ for composition). d. Block the transferred membrane with 5% non-fat milk or 5% BSA in 1X TBST for 1 h on rotation. e. Discard the blocking solution and incubate the membranes with primary antibody for 24 h at 4 ◦ C. f. The following day, wash the membranes with 1X TBST 3 times for 5 min each. ..

    Article Title: Molecular and functional characterization of a novel Toll-like receptor 9-specific DNA aptamer with therapeutic potential.
    Article Snippet: Toll-like receptor 9 (TLR9) plays a pivotal role in immune activation through recognition of DNA motifs, making it a compelling target for immunomodulatory therapies.. We employed an integrative approach combining computational docking, biophysical characterization, and functional assays to identify and characterize DNA aptamers targeting TLR9.. From an initial pool of candidates, ATA2 (agonistic TLR9 aptamer) emerged as a potent TLR9 agonist, demonstrating dose-dependent induction of TNF-α production in macrophages with specificity confirmed by TLR9 antagonist inhibition.

    Article Title: Protocol for large-scale, high-yield, high-purity extracellular vesicle purification from human plasma
    Article Snippet: .. Run the gel electrophoresis by following the same protocol as reported previously (in step 32b). c. Using Biorad Mini Trans-Blot Electrophoretic Transfer Cell system (includes two gel holder cassettes, foam pads, electrodes, tank, blue cooling unit, lid with cables) and a Biorad PowerPacTM Basic Power Supply, perform wet transfer of the gel onto a Nitrocellulose membrane at a constant voltage of 30V at 4°C for 24 h (or 120V for 2 h) in a tank filled with the 1X Transfer Buffer working solution, which is freshly made by mixing 700 mL DI water and 200 mL methanol with 100 mL of 10X Transfer Buffer stock solution (refer to “ ” for composition). d. Block the transferred membrane with 5% non-fat milk or 5% BSA in 1X TBST for 1 h on rotation. e. Discard the blocking solution and incubate the membranes with primary antibody for 24 h at 4°C. f. The following day, wash the membranes with 1X TBST 3 times for 5 min each. ..

    Article Title: Accelerated NLRP3 inflammasome-inhibitory peptide design using a recurrent neural network model and molecular dynamics simulations
    Article Snippet: To extract total protein, the M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, Inc.) was used, and the amount of protein was determined using the bicinchoninic acid (BCA) assay (Sigma-Aldrich Co.). .. The western blotting procedure, which entailed gel electrophoresis and transfer, was performed using the Mini-PROTEAN Tetra Cell and Mini Trans-Blot Electrophoretic Transfer Cell System (both from Bio-Rad Laboratories, Hercules, CA, USA). ..

    Blocking Assay:

    Article Title: Protocol for large-scale, high-yield, high-purity extracellular vesicle purification from human plasma.
    Article Snippet: .. Run the gel electrophoresis by following the same protocol as reported previously (in step 32b). c. Using Biorad Mini Trans-Blot Electrophoretic Transfer Cell system (includes two gel holder cassettes, foam pads, electrodes, tank, blue cooling unit, lid with cables) and a Biorad PowerPacTM Basic Power Supply, perform wet transfer of the gel onto a Nitrocellulose membrane at a constant voltage of 30V at 4 ◦ C for 24 h (or 120V for 2 h) in a tank filled with the 1X Transfer Buffer working solution, which is freshly made by mixing 700 mL DI water and 200 mL methanol with 100 mL of 10X Transfer Buffer stock solution (refer to “materials and equipment’’ for composition). d. Block the transferred membrane with 5% non-fat milk or 5% BSA in 1X TBST for 1 h on rotation. e. Discard the blocking solution and incubate the membranes with primary antibody for 24 h at 4 ◦ C. f. The following day, wash the membranes with 1X TBST 3 times for 5 min each. ..

    Article Title: Protocol for large-scale, high-yield, high-purity extracellular vesicle purification from human plasma
    Article Snippet: .. Run the gel electrophoresis by following the same protocol as reported previously (in step 32b). c. Using Biorad Mini Trans-Blot Electrophoretic Transfer Cell system (includes two gel holder cassettes, foam pads, electrodes, tank, blue cooling unit, lid with cables) and a Biorad PowerPacTM Basic Power Supply, perform wet transfer of the gel onto a Nitrocellulose membrane at a constant voltage of 30V at 4°C for 24 h (or 120V for 2 h) in a tank filled with the 1X Transfer Buffer working solution, which is freshly made by mixing 700 mL DI water and 200 mL methanol with 100 mL of 10X Transfer Buffer stock solution (refer to “ ” for composition). d. Block the transferred membrane with 5% non-fat milk or 5% BSA in 1X TBST for 1 h on rotation. e. Discard the blocking solution and incubate the membranes with primary antibody for 24 h at 4°C. f. The following day, wash the membranes with 1X TBST 3 times for 5 min each. ..

    Incubation:

    Article Title: Changes in lipid metabolism in visceral rather than the subcutaneous adipose tissue depot attenuate metabolic disturbances in obesity-resistant mice fed a high-fat diet.
    Article Snippet: Obesity is characterized by an enlargement of white adipose tissue caused by caloric excess.. The depot-specific adaptation of white adipose tissue in individuals resistant to obesity despite a high-calorie diet is crucial for understanding the pathogenesis of obesity and related metabolic disorders.. Our aim was to characterize the metabolic and morphological state of obesity resistance and to investigate depot-specific changes in signaling pathways in epididymal visceral (eVAT) and inguinal subcutaneous (iSAT) white adipose tissue of C57BL/6J male mice on a high-fat diet (60 kcal% fats).

    Western Blot:

    Article Title: Molecular and functional characterization of a novel Toll-like receptor 9-specific DNA aptamer with therapeutic potential.
    Article Snippet: Toll-like receptor 9 (TLR9) plays a pivotal role in immune activation through recognition of DNA motifs, making it a compelling target for immunomodulatory therapies.. We employed an integrative approach combining computational docking, biophysical characterization, and functional assays to identify and characterize DNA aptamers targeting TLR9.. From an initial pool of candidates, ATA2 (agonistic TLR9 aptamer) emerged as a potent TLR9 agonist, demonstrating dose-dependent induction of TNF-α production in macrophages with specificity confirmed by TLR9 antagonist inhibition.

    Article Title: Accelerated NLRP3 inflammasome-inhibitory peptide design using a recurrent neural network model and molecular dynamics simulations
    Article Snippet: To extract total protein, the M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific, Inc.) was used, and the amount of protein was determined using the bicinchoninic acid (BCA) assay (Sigma-Aldrich Co.). .. The western blotting procedure, which entailed gel electrophoresis and transfer, was performed using the Mini-PROTEAN Tetra Cell and Mini Trans-Blot Electrophoretic Transfer Cell System (both from Bio-Rad Laboratories, Hercules, CA, USA). ..



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